Coding

Part:BBa_K2130012:Design

Designed by: Ooi Kean Hean   Group: iGEM16_NTU-Singapore   (2016-10-14)


∆HNH Sp-dCas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 251
    Illegal BglII site found at 1188
    Illegal BamHI site found at 2006
    Illegal XhoI site found at 3154
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2350
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2496
    Illegal BsaI site found at 3350
    Illegal BsaI.rc site found at 1411


Design Notes

Assembled via Gibson


Source

Addgene

References