Coding
Part:BBa_K2130012:Design
Designed by: Ooi Kean Hean Group: iGEM16_NTU-Singapore (2016-10-14)
∆HNH Sp-dCas9
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 251
Illegal BglII site found at 1188
Illegal BamHI site found at 2006
Illegal XhoI site found at 3154 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2350
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2496
Illegal BsaI site found at 3350
Illegal BsaI.rc site found at 1411
Design Notes
Assembled via Gibson
Source
Addgene